Composition
- Crystal violet 2.0 g
- Basic fuchsin 0.05 g
- Ethyl alcohol 95% 10.0 ml
- Crystallized phenol* 10.0 ml
* Melted in water bath at 56 °C under a fume hood.
Procedure
- Mix the reagents thoroughly
- Leave to stand overnight
- Add 100 ml distilled water
- Stir well and filter.
The solution must be sheltered from sunlight and stored in a tightly sealed brown glass bottle. When used, do not shake the bottle, but take the top layer of the solution with a pipette. For use, see: “Microscopic examination with stains for temporary (wet mount) preparations”.