Composition
- Iodine crystals 1 g
- Potassium iodide (KI) crystals 2 g
- Distilled water 100 ml
Procedure
- Dissolve the potassium iodide in 10-20 ml distilled water;
- Slowly add iodine crystals to get a homogeneous solution;
- Add the remaining water, stir well and filter.
This solution is quite stable and keeps its staining properties for about one month if stored in a tightly sealed brown glass bottle sheltered from sunlight. A ready to use 1% iodine solution stabilized with PVP (polyvinyl-pyrrolidone) is commercially available and has superior stability (1 year). For use, see: “Microscopic examination with stains for temporary (wet mount) preparations”.