REAGENTS

(R5) Schaudinnís fixative

Saturated solution of mercuric chloride (HgCl2)

Procedure

-     Dissolve 10 g HgCl2 in 100 ml warm (not boiling) distilled water;

-     Leave to cool (mercuric chloride crystals deposit);

-     Filter off the clear supernatant;

-     Store in a sealed glass bottle until use.

Schaudinn's stock solution

Composition

-     Saturated mercuric chloride solution                  2 volumes

-     95% ethanol                                                     1 volume

-     Mix well.

Schaudinnís working solution

-       Immediately before use, add  5.0 ml glacial acetic acid to 95 ml of stock solution

-       Mix well.