REAGENTS

(R34) Field stain

Field stain A

-     Methylene blue                                                     0.80 g

-     Azur 1                                                                 0.50 g

-     Na2HPO4                                                             5.00 g

-     KH2PO4                                                               6.25 g

-     Distilled water                                                     500 ml

Procedure

-     Dissolve salts in hot distilled water;

-     Let cool and add stains;

-     Let stand for 24 hours;

-     Filter over blotting paper;

-     Store at room temperature out of the sunlight.

Stable for about 12 months.

Ready to use Field solution A is commercially available.