(R31) Modified trichrome stain for microsporidia


-     Chromotrope 2R                                                    6.0 g

-     Fast green FCF                                                    0.15 g

-     Phosphotungstic acid                                            0.70 g

-     Glacial acetic acid                                                 3.0 ml

-     Distilled water                                                   100.0 ml

Compared to the trichrome solution of Wheatley, the concentration of chromotrope 2R here is 10-times higher.  For the procedure, see Trichrome solution