Solution A
- Basic fuchsin 4 g
- Ethanol 95% 20 ml
Dissolve stain in ethanol and mix well.
Solution B
- Phenol crystals melted in water bath (56? C) 8 ml
Procedure
- Mix solutions A and B;
- Add 100 ml distilled water, stir well;
- Let stand for 1-2 days.
- Filter and store in a dark glass bottle.
Stable for an indefinite period.