(R28) Kinyoun’s stain (cold method, for coccidia)

Solution A

-     Basic fuchsin                                                            4 g

-     Ethanol 95%                                                         20 ml

Dissolve stain in ethanol and mix well.

Solution B

-     Phenol crystals melted in water bath (56? C)              8 ml


-     Mix solutions A and B;

-     Add 100 ml distilled water, stir well;

-     Let stand for 1-2 days.

-     Filter and store in a dark glass bottle.

Stable for an indefinite period.