REAGENTS

(R25) Ziehl-Neelsen modified acid-fast stain (hot method, for intestinal coccidia)

Solution A

-      Basic fuchsin                                                         0.3 g

-      Ethanol 95%                                                         10 ml

Dissolve fuchsin in ethanol, and mix well.

Solution B

-      Phenol crystals                                                       5.0 g

-      Distilled water                                                   100.0 ml

Dissolve phenol crystals in distilled water in a water bath at 56 C under a fume hood.

Carbol-fuchsin working solution

Mix solutions A and B.  Store at room temperature. The solution is stable for at least 1 year.