(R18)  Heidenhain’s hematoxylin solution A

Composition

-     Hematoxylin crystals                                                10 g

-     95% ethanol                                                      1000 ml

Procedure

-     Dissolve hematoxylin crystals in alcohol in a clear glass container;

-     Seal and leave to oxidize (or “ripen”) in daylight (not direct sunlight) for at least 6-8 weeks. Frequent shaking hastens the ripening process. A longer exposure (months, up to two years) increases the staining properties of the solution.