Fig.1. SAF fixed stool specimen: Entamoeba histolytica/dispar trophozoite with elongated pseudopod fixed during the movement. With this fixative the nuclear structures are well differentiated and allows the correct identification of amoebae trophozoites.
Fig.2. SAF fixed stool specimen: Entamoeba histolytica/dispar trophozoite, rounded form.
Fig.3. SAF fixed stool specimen: Entamoeba coli trophozoite unstained.
Fig.4. SAF fixed stool specimen: an Entamoeba coli trophozoite stained with iodine. The cytoplasm contain numerous food vacuoles, bacteria and other fecal debris.